Article citationsMore>>
Garcia-Prieto, C., Riaz Ahmed, K.B., Chen, Z., Zhou, Y., Hammoudi, N., Kang, Y., Lou, C., Mei, Y., Jin, Z. and Huang, P. (2013) Effective Killing of Leukemia Cells by the Natural Product OSW-1 through Disruptio of Cellular Calcium Homeostasis. Journal of Biological Chemistry, 288, 3240-3250.
https://doi.org/10.1074/jbc.M112.384776
has been cited by the following article:
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TITLE:
OSW-1 Induced Apoptosis in Hepatocellular Carcinoma through Generation of ROS, Cytochrome C and Noxa Activation Independent of p53 with Non-Activation of Caspase-3
AUTHORS:
Xiaochen Liu, Jingchao Liang, Jichun Jin, Haiyang Li, Bosheng Mei, Xinglin Jin
KEYWORDS:
OSW-1, Hepatocellular Carcinomas, ROS, Cytochrome C, Mitochondrial Death Pathway
JOURNAL NAME:
Chinese Medicine,
Vol.8 No.1,
March
14,
2017
ABSTRACT: Aim: To study the antitumor mechanism of OSW-1 in hepatocellular
carcinoma. Materials and Methods: The expression profiling microarray was carried
out to extract RNA from SK-Hep-1 which suffered from OSW-1. ρ0-SK-Hep-1 was maintained SK-Hep-1 in MEM
containing 100 μg/L ethidium bromide (EB), 1 mM sodium pyruvate and 50
μg/ml uridine for 40 days. Then confirmed
COX-I and COX-II of mitochondrial DNA were knocked out. Cells suffered
from OSW-1 or doxorubicin. Then cells were washed twice with cold PBS and incubated with DCFH-DA. Fluorescent signal was recorded by using Infinite 200 Pro multimode Plate
readers. Results: OSW-1 elevates generation of ROS and Cytochrome C
which are associated with the induction of apoptosis in SK-Hep-1 cells. We also
demonstrate that OSW-1 does not depend on p53
to up-regulate the BH3-only protein Noxa. What is more noteworthy that
the Caspase-9 and FADD are down-regulated in above process. Conclusion: OSW-1 induced special apoptosis is different from
the mitochondrial death pathway and the death receptor pathway and final
result is not Caspase family’s activating.
This provides a novel theory that nonmalignant cells are significantly
less sensitive to OSW-1 than cancer cell lines.