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Raal, F.J., Stein, E.A., Dufour, R., Turner, T., Civeira, F., Burgess, L., Langslet, G., Scott, R., Olsson, A.G., Sullivan, D., Hovingh, G.K., Cariou, B., Gouni-Berthold, I., Somaratne, R., Bridges, I., Scott, R., Wasserman, S.M. and Gaudet, D. (2015) PCSK9 Inhibition with Evolocumab (AMG 145) in Heterozygous Familial Hypercholesterolaemia (RUTHERFORD-2): A Randomised, Double-Blind, Placebo-Controlled Trial. Lancet, 385, 331-340.
https://doi.org/10.1016/S0140-6736(14)61399-4

has been cited by the following article:

• TITLE: The New Molecular Entity Evolocumab, One Kind of PCSK9 Inhibitor, Reduce Plasma Small Size LDL-Cholesterol Levels by Using a New Standardized Method of Measuring LDL Size

  AUTHORS: Ikuo Inoue, Ryo Kubota, Shohan Yanagi, Masumi Akita, Takanari Nakano, Shigehiro Katayama, Akira Shimada, Mistuhiko Noda

  KEYWORDS: PCSK9 Inhibitor, Evolocumab, LDL Heterogeneicity, Small Size LDL, 3% PAGE, 2% - 16% GGE

  JOURNAL NAME: Open Journal of Molecular and Integrative Physiology, Vol.7 No.1, February 28, 2017

  ABSTRACT: Aims: There has been no evidence on the effects of evolocumab, protein convertase subtilisin/kexin type 9 (PCSK9) inhibitor, on small size LDL. We observationally investigated the efficacy and side effects of evolocumab on the LDL subfraction particle diameter using PAGE system for lipoprotein analysis. Methods: We defined 30 patients with high-risk hyperlipidemia. As for analysis of LDL subfraction profile, we used polyacrylamide gel electrophoresis three methods: 1) 3% nondenatured poly-acrylamide gel electrophoresis method (3%PAGE), 2) 2% - 16% nondenatured poly-acrylamide gradient gel electro-phoresis method (2% - 16% GGE) and 3) 2.7% - 5% GGE. Evolocumab 140 mg/day administered together with statin significantly improved serum total cholesterol (TC), triglyceride (TG), high-dense lipoprotein-cholesterol (HDL-C), and LDL-C after four-week treatment. Results: TC, TG, HDL-C and LDL-C levels were improved by, respectively, 33%, 20%, 10%, and 54%. The mean LDL size significantly increased from 25.6 ± 0.4 nm to 26.4 ± 0.8 nm. The small dense LDL-cholesterol (sdLDL-C), large buoyant LDL-cholesterol (lbLDL-C), and mid-band lipoprotein-cholesterol were reduced, respectively. Therefore, the preliminary study on this paper can be the first step into a new insight on the world of lipid metabolism. Conclusion: Short-term administration of evolocumab addedons to statin therapy, significantly reduced small size LDL levels.