TITLE:
Fermented Ethnic Medicine (Pueraria mirifica) Designed by Lactobacillus Regulated Leukocyte Subsets via Activation of Complement Components
AUTHORS:
Yoshiichiroh Mastuba, Kazuhiro Okamoto, Yuma Kato, Nobuo Yamaguchi, Sumiyo Akazawa-Kudoh
KEYWORDS:
Family Pueraria, Fermentation, Lactobacilli, Cancer Chemotherapeutic Agent, Immune-Compromised Host, Lymphocyte, Granulocyte, Ant-Oxidative Activity, Complement, Alternative Complement Activation
JOURNAL NAME:
Open Journal of Rheumatology and Autoimmune Diseases,
Vol.7 No.1,
February
10,
2017
ABSTRACT: A plant fermentation was carried out by Lactobaccilli against the Rhizome from Pueraria milifica (f-PMF).
This material was evidenced by safe in animal toxic study. The main aim of this
study was to revise the traditional way of hot water extraction to fermentation
so as to use up the original material and finding new activity. We tried to
show the new activity through phytoestrogen and immune-competent cells from the
host that administrated either of original remedy and the new fermented sample,
plus activated water SRE. In mice, compromised host was prepared by cancer
chemotherapeutic agent (Mitomycin-C). After administration of f-PMF to immno-suppressed animals, the
effects by both samples were augmented by lymphocyte in number and functions,
macrophage activities, anti-oxidative activity. However, the intense of effect
was much more by fermented one but not by conventional one. The anti-oxidative
assay was also carried out ex-vivo system by peritoneal macrophage that we proposed as suitable system for
evaluating anti-oxidative assay. In our clinical study by 20 healthy volunteers,
granulocyte and lymphocyte ratio was regulated as neutral in peripheral white
blood cells, increasing one, two and three weeks after the administration of f-PMF. We have found the significant
regulation of blood chemical factors that were important makers for the
lifestyle-related diseases. The mechanism of augmentation by probing directory
with immuno-electrophoretic method, generating new complement component,
especially found by alternative pathway of complement. So we discussed the
process concerning designed f-PMF
molecule for activation of complement component and bound for the biological activity
of each physical component. In a limited condition, fewer numbers of volunteers,
the breast size was tending to increase along with the administration time.
Including these evidences, we discussed the
possibility of this traditional ethnic medicine, originally found and spread in
the highland area in Thailand and Myanmar.