TITLE:
Analysis of the Liver of Fish Species Prochilodus lineatus Altered Environments, Analyzed with ImageJ
AUTHORS:
Vanessa Ellen Wendt Campos, Bruno Fiorelini Pereira, Dimítrius Leonardo Pitol, Rebeca Mamede da Silva Alves, Flavio Henrique Caetano
KEYWORDS:
Liver, Glycogen, Bile, Prochilodus lineatus
JOURNAL NAME:
Microscopy Research,
Vol.5 No.1,
January
22,
2017
ABSTRACT: The fish Prochilodus lineatus (Characiformes, Prochilodontidae), in addition to being a good
bioindicator, is also of economic and ecological importance with a broad distribution
in the neotropics. Ecotoxicology examines the interaction between environmental
chemistry and biota; and in this study we assess alterations of bile and
glycogen levels in the fish liver, organ responsible for detoxification,
biotransformation and storing nutrients, such as glycogen, and for secreting
bile. Fish were separated in three groups to examine
the damage caused by the exposure to waters from Lago Azul-Rio Claro-SP and containing diluted biodegradable
detergents in comparison to a control group (chlorinated water from an artesian
well of UNESP-Campus Rio Claro). A histological analysis was performed on HE
and PAS stained sections. The identification of structural changes and the assessment of the area occupied by bile and glycogen were
carried out with the software ImageJ, showing that the liver was affected morphologically (cell vacuolization,
peripherals nuclei, for example) and problems in bile release and production
and storage of glycogen.