TITLE:
Root Extracts (Gymnadenia orchidis Lindl) Facilitated Rapid Synthesis of Fluorescent Silver Nanoparticles (Ag-NPs) for Various Biological Applications
AUTHORS:
Shilpi Show, Chetana Ghosal, Brajadulal Chattopadhyay
KEYWORDS:
Green Synthesis, Gymnadenia orchidis Lindl, Silver Nanoparticles, DPPH, Photo-Catalytic, Antibacterial
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.8 No.1,
January
22,
2017
ABSTRACT: One pot rapid and green bio-synthesis of stable fluorescent silver
nanoparticles (Ag-NPs) from silver nitrate solution using root extract of Gymnadenia orchidis Lindlat at ambient temperature is demonstrated productively. Surface
Plasmon resonance of the synthesized Ag-NPs
was shown to occur at 439 nm and two sharp fluorescence peaks at 610 and 780 nm
were observed. FTIR study publicized the presence of chemically functional groups in the
root extract surrounding the Ag-NPs, acting as stabilizers. XRD and FESEM analysis revealed that
Ag-NPs were face centred cubic structure being
spherical in shape with an average particle size of 28 ± 2 nm (n = 100). The
stability of Ag-NPs in neutral pH environment was confirmed by Zeta
Potential (ζ) behaviour. The Ag-NPs
showed superior antioxidant activity. The notable photo-catalytic activity of
biosynthesized Ag-NPs attributed to the existence of some proteins, responsible
for degradation of methylene blue dye. Furthermore, Ag-NPs were found to
exhibit a significant antibacterial effect against gram positive Staphylococcus
aureus (S. aureus) bacteria.