TITLE:
Fast Detection of Auxins by Microplate Technique
AUTHORS:
Julia Cecilia Anguiano-Cabello, Alberto Flores-Olivas, Yisa María Ochoa-Fuentes, Roberto Arredondo-Valdés, Victor Olalde-Portugal
KEYWORDS:
Auxin, Microplate, Bacillus subtilis
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.2,
January
20,
2017
ABSTRACT: Plant growth promotion indole-3-acetic acid (IAA) is
the most abundant natural auxin that plays diverse roles in plant growth,
development and plant immunity. Perturbing auxin homeostasis appears to be a
common virulence mechanism, as many pathogens can synthesize auxin-like
molecules. In other hand, the addition of plant growth promotion rhizobacteria (PGPR) that are able to produce auxins promotes plant growth and provides protection against pathogens. Techniques as high
performance liquid chromatography (HPLC) and gas chromatography (GC) are used
to quantify auxins produced by microorganism and plants at high precision and
sensitivity, even though
those techniques are expensive and require a big number of solvents. For
these reasons, the
aim of the present study was to develop a fast microplate technique for auxin detection, in Bacillus subtilis strains using
salkowski reagent. For auxin quantification, calibration curves were done with alcohol, landy
medium and water and the R2 were calculated. The microplate
techniques were able to quantify auxin production by B. subtillis stains.