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National Committee for Clinical Laboratory Standards (2003) Methods for Dilution Antimicrobial Susceptibility Test for Bacteria That Grow Aerobically: Approved Standard. 6nd Edition, Wayne (NCCLS Document M7-A6; NCCLS, 940).

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• TITLE: Characterization of the Biological Potential of the Essential Oils from Five Species of Medicinal Plants

  AUTHORS: Danubia Aparecida Carvalho Selvati Rezende, Rafaela Vieira Souza, Maisa Lamounier Magalhães, Alex Rodrigues Silva Caetano, Marcos Schleiden Sousa Carvalho, Ellen Cristina de Souza, Luiz Gustavo de Lima Guimarães, David Lee Nelson, Luís Roberto Batista, Maria das Graças Cardoso

  KEYWORDS: Natural Products, Antioxidant, Antibacterial Activity

  JOURNAL NAME: American Journal of Plant Sciences, Vol.8 No.2, January 20, 2017

  ABSTRACT: Essential oils from Mentha piperita, Cymbopogon citratus, Rosmarinus officinalis, Peumus boldus and Foeniculum vulgare were extracted by hydrodistillation, chemically characterized and quantified by GC/MS and GC/FID. The principal componentes in the essential oil of M. piperita (carvone and limonene), C. citratus (geranial, neral and myrcene), R. officinalis (1,8-cineole, camphor and α-pinene), P. boldus (α-terpinyl formate, p-cymene and 1,8-cineole) and F. vulgare (methyl chavicol, limonene and fenchone) were identified. The oils were tested for antioxidant activity employing the DPPH (2,2-diphenyl-1-picryl-hydrazyl) radical-capture method, the oxidation of the β-carotene/linoleic acid system, and the ABTS radical-capture method. The essential oils were not effective. The antioxidant activities of the oils were determined by the hydroxyl method, and the highest activity (62.80%) was observed with the essential oil from M. piperita. Activities of 1.54 and 1.82 μg μg-1, respectively, were observed for the essential oils from C. citratus and P. Boldus using the phosphomolybdenum method. No activity was observed with respect to reducing power. The essential oil from C. citratus was effective in inhibiting the growth of L. monocytogenes (15.63 mg·L-1), S. choleraesuis (15.63 mg·L-1) and E. coli (31.25 mg·L-1), and the essential oil from P. boldus inhibited the growth of S. aureus (62.5 mg·L-1). No essential oil inhibited the growth of P. aeruginosa. A low antioxidant activity and a promising antibacterial activity were observed for these essential oils.