TITLE:
Chromosomal Location of a Recessive Red-Eye Mutant Gene in the Brown Planthopper Nilaparvata lugens (Stål) (Insecta: Hemiptera)
AUTHORS:
Jirapong Jairin, Phikul Leelagud, Adisak Pongmee, Kanuengnij Srivilai
KEYWORDS:
Brown Planthopper, Bulked Segregant Analysis, Linkage Map, Red-Eyed Mutant, Simple Sequence Repeat
JOURNAL NAME:
Advances in Entomology,
Vol.5 No.1,
January
19,
2017
ABSTRACT: The color of compound eyes is an important
biological characteristic of insects. A red eye color mutation is commonly
found in the brown planthopper (BPH), Nilaparvata lugens (Stål)
(Hemiptera: Delphacidae), a serious insect pest of rice in tropical and
temperate Asia. The genetic inheritance and physiological effect of the eye
color mutation in the BPH have been studied, but the location of a red gene controlling the red eye mutant phenotype on a chromosome has not been
elucidated. In this study, simple sequence repeats (SSRs), together with bulked
segregant analysis (BSA), was performed to identify and map the location of the red gene. A total of 387 SSR markers distributed throughout the BPH
autosome were used to survey two bulked DNA samples. Samples were generated
from 29 brown-eyed and 29 red-eyed individuals derived from an F2 generation of a cross between brown-eyed wild type and red-eyed mutant
colonies. The SSR marker BM20 was shown to be associated with the red eye
mutant phenotype. Ninety-five offspring of the F2 generation were
then used to map the gene. The present study constitutes the discovery of the
location of the red gene, which may lead to the acquisition of the
genetic determinant of the compound eye color mutation in BPH.