TITLE:
Diagnostic and Prognostic Significance of Histidine-Rich Glycoprotein in Acute Lymphoblastic Leukemia
AUTHORS:
Manal Basyouni Ahmed, Ebtehal Almogbel, Iman Khirry, Sara Hassan, Tarek Salem, Aliaa Saeed
KEYWORDS:
Acute Lymphoblastic Leukemia, Histidine-Rich Glycoprotein and Serum Markers
JOURNAL NAME:
Open Journal of Blood Diseases,
Vol.7 No.1,
December
30,
2016
ABSTRACT: Background: Histidine-rich glycoprotein (HRG), a multifunctional plasma protein, has a regulatory role in homeostasis, angiogenesis, and immunity; which in turn could greatly affect tumor control and metastasis. Objectives: To assess the possible role of HRG in acute lymphoblastic leukemia (ALL) tumorgenesis and follow-up. Design and Methods: HRG was quantitatively measured in serum by ELISA and its expression was assessed by real-time PCR (qPCR) in 35 patients with ALL and compared to same 25 ALL patients after induction therapy and 30 age and sex matched healthy control subjects. Results: HRG-serum protein (at cutoff value 63.55 pg/ml) and HRG-RNA (at cut-off value 0.955) were positive in all ALL patients before therapy, but in only 76% after therapy for HRG-protein and 60% for HRG-RNA and they could not be detected in the control group; P