TITLE:
Comparison between Two Ethanolic Solutions for 3’-Deoxy-3’-[18F]Fluorothymidine Elution
AUTHORS:
Leonardo T. C. Nascimento, Marina B. Silveira, Soraya M. Z. M. D. Ferreira, Juliana B. Silva
KEYWORDS:
[18F]FLT, Synthesis, Quality Control, Formulation, Elution, Radiopharmaceutical
JOURNAL NAME:
Advances in Chemical Engineering and Science,
Vol.7 No.1,
December
21,
2016
ABSTRACT: 3’-Deoxy-3’-[18F]Fluorothymidine—[18F]FLT is a Positron Emission Tomography (PET) tracer which
has been used for noninvasive assessment of proliferation activity in several
types of cancer. During the past few years, some novel approaches for [18F]FLT
synthesis have been developed, mainly focused on optimization of reaction
conditions and purification methods. The present study reports the use of two
different eluents in the final step of [18F]FLT production and the
evaluation of its effect on radiochemical yield and product quality. The first
eluent evaluated was water: ethanol
(90:10, v/v), commercially available, and the second was NaCl 0.9% (saline): ethanol (92:8, v/v). The mean of the corrected
radiochemical yields corresponded to 27% ± 7% for
elution with water and ethanol and to 23% ± 3% for
elution with saline and ethanol, which could indicate that the eluent solutions
have similar elution strength. Besides, quality control results were in
accordance with the requirements and demonstrated that there was no significant
difference between both formulations. Considering that pharmaceutical
preparations containing ethanol should be preferentially diluted with saline to
avoid hemolysis, the eluent saline:ethanol (92:8, v/v) was chosen for [18F]FLT
extraction and final formulation.