TITLE:
Physiological and pathophysiological roles of the electrogenic Na+-HCO3– cotransporter NBCe1
AUTHORS:
George Seki, Hideomi Yamada, Shoko Horita, Masashi Suzuki, Osamu Yamazaki, Wim Van Paesschen, Sung-Sun Yang, Shih-Hua Lin
KEYWORDS:
NBCe1; pRTA; Migraine; Academia; W516X Knockin Mice
JOURNAL NAME:
Open Journal of Molecular and Integrative Physiology,
Vol.1 No.2,
August
22,
2011
ABSTRACT: The electrogenic Na+-HCO3– cotransporter NBCe1 encoded by SLC4A4 gene plays essential roles in the regulation of intracellular/extracellular pH. Three NBCe1 variants are thought to mediate distinct physiological roles with different modes of transport stoichiometry. Homozygous inactivating mutations in NBCe1 cause the isolated proximal renal tubular acidosis (pRTA) invariably associated with ocular abnormalities. Functional analyses indicate that more than 50% reduction in NBCe1 activity may be required to induce severe acidemia. Some of the pRTA- related NBCe1 mutations, which show defective me-mbrane expression in mammalian cells, are also associated with migraine. Dysregulation of local pH in brain due to the loss of NBCe1 activity in astrocytes may underlie this association. Two types of NBCe1 deficient animals, NBCe1 knockout and W516X knockin mice, have been reported. Both of them show severe acidemia and early lethality unless they are treated with alkali. In isolated renal proximal tubules from W516X knockin mice, both NBCe1 activity and the rate of bicarbonate absorption are severely reduced, confirming the essential role of NBCe1 in bicarbonate absorption from this nephron segment. In this review, we summarize the recent data about physiological and pathophysiological roles of NBCe1 in health and diseases.