TITLE:
Agromorphological and Molecular Characterization of Sesamum indicum L.—An Oil Seed Crop
AUTHORS:
Tapaswini Hota, Chinmaya Pradhan, Gyana Ranjan Rout
KEYWORDS:
Sesamum, PCR Based Marker, Phylogeny, Molecular Profiling, Agro-Mophological Traits
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.17,
December
8,
2016
ABSTRACT: Sesamum indicum L. (family: Pedaliaceae) is an economically important oil seed
crop grown in tropical and sub-tropical countries. It is widely used in food, nutraceutical,
pharmaceutical industries. Sesamum is widely distributed in all the climatic
stages and great diversity. The exploration of genetic diversity is a pre-requisite for
genome organization in the landraces and the related domesticated ones. Agromorphological
and molecular markers were used to assess the identification of 33 Sesamum genotypes and determination of the genetic relationships among these
genotypes. Out of 30 Inter-Simple Sequence Repeat (ISSR) primers tested, 18 primers
produced 114 detectable fragments, of which 97 (85.08%) were polymorphic across
the varieties. Molecular profiling could be solely used for their identification of
genotypes. Genetic relationships among these genotypes were evaluated by generating
a similarity matrix based on the Jacard’s coefficient and the Unweighted Pair
Group Method with Arithmetic Average (UPGMA) dendogram. The results showed
a clear cut separation of the 33 genotypes and were in broad agreement with the
morphology. Both molecular and morphological markers will be useful for preservation
of the germplasm as well as breeding program.