TITLE:
Novel Protease from Aspergillus tamarii URM4634: Production and Characterization Using Inexpensive Agroindustrial Substrates by Solid-State Fermentation
AUTHORS:
Osmar Soares da Silva, Rodrigo Lira de Oliveira, Cristina Maria Souza-Motta, Ana Lúcia Figueiredo Porto, Tatiana Souza Porto
KEYWORDS:
Protease, Aspergillus tamarii, Biochemical Characterization, Solid-State Fermentation, Agroindustrial Waste
JOURNAL NAME:
Advances in Enzyme Research,
Vol.4 No.4,
December
2,
2016
ABSTRACT: This study reports the
protease production from Aspergillus tamarii using agroindustrial residues as substrate for solid-state fermentation (SSF)
and biochemical characterization. The highest protease production was obtained
using wheat bran as substrate at 72 h fermentation with maximum proteolytic
activity of 401.42 U/mL, collagenase of 243.0 U/mL and keratinase of 19.1 U/mL.
The protease exhibited KM = 18.7 mg/mL and Vmax = 28.5 mg/mL/min.
The optimal pH was 8.0 and stable in a wide pH range (5.0 - 11.0) during 24 h.
The optimum temperature was 40°C. The
proteolytic activity was inhibited by Cu2+ (33.98%) and Hg2+ (22.69%). The enzyme was also inhibited by PMSF (65.11%), indicating that is a
Serine Protease. These properties suggest that alkaline protease from A. tamarii URM4634 is suitable for
application in food industries and leather processing. Additionally, the
present findings opened new vistas in the utilization of wheat bran and other
effective agroindustrial wastes as substrates for SSF.