TITLE:
Point-of-Care Testing Using Three Dimensional Optical Biosensor Based on Microfluidic Technology
AUTHORS:
Chunxiu Liu, Haoyuan Cai, Jian Jia, Tianyang Cao, Tong Li, Tianjun Ma, Chang Liu
KEYWORDS:
Point-of-Care Testing (POCT), Three Dimensional Optical Chip, hs-CRP
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.4 No.12,
December
1,
2016
ABSTRACT: We have presented a three dimensional optical protein chip that fulfills the demanding for point-of-care diagnostics in terms of ease-of-use (one step assay), miniaturization (5 μl). The artful combination of magnetic nanoparticles on chip and total internal reflection imaging (TIRI) technology permits the sensitive and rapid detection of hs-CRP (high-sensitivity C-reactive protein). The whole test was complete within 10 min using “all in one step” assay with a limit of detection of 0.1 ng/mL hs-CRP. The measuring range for hs-CRP could be extended to 10 ng/mL. The chip can also be used to detect more parameters in blood samples.