TITLE:
In-Vitro Production of Anthocyanin in Sesbania grandiflora (Red Katuray) as Influenced by Varying Concentrations of 2,4-D and BA Added on MS Medium
AUTHORS:
Donata A. Largado-Valler
KEYWORDS:
Anthocyanin, In Vitro, 2, 4-D, BA
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.15,
November
18,
2016
ABSTRACT: The study aimed to determine the anthocyanin content in callus cultures of Sesbania
grandiflora L. (Red Katuray) using petal explants as influenced by 2,4-D and BA. The
specific objective of this study is to determine the effect of plant growth regulators
specifically auxin (2,4-D) and BA (Benzyladenine) on Murashige and Skoog’s (MS)
medium in anthocyanin production. Earliest callus initiation was noted in 2.5 ppm to
5.0 ppm 2,4-D without BA and 2.5 ppm 2,4-D with 2.5 ppm BA. Same treatment
combination had the highest degree of callus formation and obtained the greatest
percentage of callus formation 2.5 ppm 2,4-D without BA and 2.5 ppm 2,4-D with
2.5 ppm BA. The heaviest weight of callus was registered at 2.5 ppm 2,4-D without
BA. Significantly higher anthocyanin content was noted in 2.5 ppm to 5.0 ppm 2,4-D
without or with 2.5 ppm BA, with 2.5 and 5.0 ppm BA and with 5.0 ppm 2,4-D and
2.5 ppm BA. There is a positive relationship between callus formation and anthocyanin
production. Results showed that treatments that promoted significantly greater
callus formation e.g. 2.5 ppm 2,4-D alone or in combination with 2.5 ppm BA also
produced the highest anthocyanin content.