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Young, D.A., Gavrilov, S., Pennington, C.J., Nuttall, R.K., Edwards, D.R., Kitsis, R.N. and Clark, I.M. (2004) Ex-pression of metalloproteinases and inhibitors in the dif-ferentiation of P19CL6 cells into cardiac myocytes. Bio-chemical and Biophysical Research Communications, 322, 759-765.

has been cited by the following article:

• TITLE: Applicability of the P19CL6 cells as a model of cardiomyocytes – a transcriptome analysis

  AUTHORS: Iraj Khodadadi, Nick J. Plant, Vassilis Mersinias, Alfred E. Thumser

  KEYWORDS: Gene expression; Cardiomyocyte; P19CL6 Cell-line

  JOURNAL NAME: Health, Vol.2 No.1, January 15, 2010

  ABSTRACT: The P19CL6 cell-line, a clone of the P19 mouse embryonal carcinoma cell-line, has been exten-sively used as a model for cardiomyocytes as these cells can be differentiated into a cardio-myocyte phenotype upon incubation with di-methyl sulfoxide. Uniquely, these cells can be observed to “beat” when monitored by mi-croscopy. We started investigating the response of P19CL6 cells to fatty acids, but highly vari-able results lead us to investigate the phenotype of the P19CL6 cells in more depth. In this study we demonstrated that the P19CL6 cells are re-sponsive to adrenaline, but loose the “beating” phenotype after 16 passages in culture. Analysis of specific mRNA transcripts indicated that the P19CL6 cells express both cardiac- and skeletal muscle-specific genes, while global analysis of microarray data showed clear differences be-tween the P19CL6 cells and cardiac tissue of embryonic or adult origin. In conclusion, our observations suggest caution in the use of the P19CL6 cells as a model of cardiomyocytes unless rigorous validation for the intended analysis has been undertaken.