TITLE:
Survival, Proliferation and Cell Cycle of Swine Fibroblast after Infection with Salmonella enterica
AUTHORS:
Mizuki Masuda, Yijie Guo, Kengo Kuroda, Jun Xu, Hiroshi Yoneyama, Tomokazu Fukuda, Bernard Mudenda Hang’ombe, Katsuya Okuno, Junko Nishimura, Emiko Isogai
KEYWORDS:
Salmonella enterica, Fibroblast, Swine, Cell Death, Cell Cycle
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.13,
November
9,
2016
ABSTRACT: Food-borne salmonellosis continues to be a major health concern worldwide. Carry-contamination of Salmonella frequently occurs in meat production. We focused on cell dynamics of swine fibroblasts after infection with Salmonella enterica serovar Enteritidis and Typhimurium, because fibroblast can be a target cell for Salmonella latent infection. It was found that both S. Enteritidis and S. Typhimurium were able to adhere and invade to swine fibroblasts. The proliferations in fibroblasts were different between each serovar. S. Enteritidis reached to the maximum at 24 hr after infection while S. Typhimurium did not. In addition, the decrease in the G0/G1 phase cells and increase in G2/M phase cells on the fibroblast were observed by both Salmonella infection. Cell death including apoptosis in the cells was inhibited by the infection of Salmonella. These results suggest that nontyphoidal Salmonella can survive for the long term by modifying bacterial cell proliferation and preventing cell death of host cells.