TITLE:
Rapid Screening of Recombinant Plasmids by Direct Colony Quantitative Real-Time PCR
AUTHORS:
Lei Hou, Xiuying Zhang, Yang Li, Shuai Chen, Hongyi Qu, Jiazhi Yu, Lianhai Zhang, Ziyi Fan
KEYWORDS:
qPCR, Colony PCR, Plasmid Screening
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.7 No.10,
October
31,
2016
ABSTRACT: Quantitative real-time PCR (qPCR) was applied to rapid screening of positive plasmid clones. Insert-specific primer pairs were used in qPCR colony screening, and false positive colonies could easily be distinguished from true positive ones by comparing their Ct values. In addition, qPCR is particularly suitable when amplicon is small (