TITLE:
Analysis on Genetic Diversity of Radix Astragali by ISSR Markers
AUTHORS:
Yaling Liu, Pengfei Zhang, Ru Zhang, Meiling Song, Fengbo Liu, Wenquan Wang, Junling Hou
KEYWORDS:
Radix Astragali, ISSR, Genetic Diversity
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.7 No.10,
October
20,
2016
ABSTRACT: Radix Astragali has been an important traditional Chinese herbal medicine for over
2000 years. It is derived from two plant species, namely, Astragalus mongholicus [Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao] and Astragalus
membranaceus [Astragalus membranaceus (Fisch.) Bge.] (Leguminosae ), according
to the Pharmacopoeia of the People’s Republic of China. In this study, the
genetic diversity and genetic relationships of Radix Astragali in China were analyzed
by Inter-Simple Sequence Repeat (ISSR) markers. A total of 25 highly polymorphic
ISSR primers were selected to amplify 95 Radix Astragali samples. Among 273 DNA
bands amplified, 213 are polymorphic (percentage of polymorphic bands: 78%). The
average value of the amplified bands was 10.9 for each primer, and the number varied
from 4 to 20. The genetic diversity of the 95 Radix Astragali samples was analyzed
by using POPGENE 1.32 software. The Nei’s genetic diversity index (h) and
Shannon’s information index (I ) were 0.3590 and 0.5308, respectively, which indicated
the abundant genetic diversity of Radix Astragali . The level of genetic diversity
in A. membranaceus (h: 0.3109, I : 0.4657) was slightly lower than that in A. mongholicus (h: 0.3364, I : 0.4969). Considering the average genetic similarity coefficient by
NTSYS analysis to cluster the A. membranaceus of nine habitats and A. mongholicus of five habitats, Radix Astragali samples were clustered into two groups according to
place of origin. This clustering is different from traditional clustering, which divides
groups according to species. Results obtained from this study will provide a theoretical
basis for the molecular study on germplasm resources of Radix Astragali .