TITLE:
The Modulatory Effect of Dietary Apostichopus japonicus on Mice with Ulcerative Colitis Induced by Trinitrobenzene Sulfonic Acid
AUTHORS:
Hongjie Shi, Hong Sun, Rong Zheng, Shangyun Lu, Fang Liu, Na Zhang, Changhu Xue, Qingjuan Tang
KEYWORDS:
Apostichopus japonicus, Ulcerative Colitis, Trinitrobenzene Sulfonic Acid, Intestinal Microbiota
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.4 No.10,
October
14,
2016
ABSTRACT: Sea cucumber is a food with nutritional benefits distributing mainly in
Asia, and Apostichopus japonicus (A. japonicus) is
a kind of sea cucumber whose quality is better than any others. However, different
processing methods make various effect on its quality. In this study, we evaluated
the protection effect of A. japonicus with different processing
methods on mice with ulcerative colitis induced by trinitrobenzene sulfonic
acid (TNBS), especially on the intestinal microflora. The expression of IFN-γ/IL-4 and IL-1β in gut, and intestinal microbiota were discussed. The results
revealed that three different processing methods of A. japonicus could decrease the expression of inflammatory cytokines, except for
the expression of IFN-γ/IL-4 treated with enzymatic, and dried A. japonicus was
the most efficient. A. japonicus could change the microbiotic imblance relatively
back to normal in terms of bacterial diversity and composition, meanwhile
increase the abundance of Bifidobacteria,
Lactobacillus and Clostridium leptum. The elements of
protein, polysaccharide in dried, instant, enzymatic A. japonicus are 73.09%, 65.06%, 57.42%
and 6.72%, 5.46%, 5.45% respectively. This
study indicated that A. japonicus have
a good improving effect on ulcerative colitis, especially on the microbiome,
and processing methods had an effect on alleviation of ulcerative colitis,
which might be associated with content of protein and polysaccharide.