TITLE:
Proteomic Analysis through Adventitious Rooting of Pinus radiata Stem Cuttings with Different Rooting Capabilities
AUTHORS:
Carolina Álvarez, Luis Valledor, Patricia Sáez, Manuel Sánchez-Olate, Darcy Ríos
KEYWORDS:
Pinus radiata, Adventitious Rooting, Protein Accumulation, Aging
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.14,
September
29,
2016
ABSTRACT: In forest production systems, vegetative propagation
of elite clones through adventitious
rooting is a common practice. In Chile, adventitious rooting is the main methodology
for vegetative reproduction of Pinus
radiata. However, the capability of produce adventitious roots in
gymnosperms decreases with aging. While it is true that some efforts have been
made to identify markers or/and regulators of the aging process and
adventitious rooting, molecular mechanisms that regulate both processes are
scarcely known, especially at protein level. This research evaluated
qualitative and quantitative changes in protein accumulation during the
adventitious rooting process of P.
radiata stem cuttings, with different rooting capabilities. Beside, an
analysis of morpho-anatomical changes was performed in stem cuttings with high
and low rooting capabilities, during the adventitious rooting process. It was
observed that juvenile 1-year-old stem cuttings rooted in a 100%, while aged
stem cuttings (3-year-old) presented only a 20% of rooting. According to the results of
differential protein accumulation, univariate and multivariate analysis
indicated that in total, 114 and 89 proteins were differentially accumulated in
juvenile and aged cuttings, respectively. Also, identification of such proteins
showed the presence of proteins related to cell wall organization and the
presence of a protein related with proper distribution of auxin PIN
transporter, both key in the new meristem formation process during adventitious
rooting.