TITLE:
Investigating the Extent of CCL4 and CCL5 Chemokine as Well as IL17 and IL23 Cytokine Gene Expression in the Patients Afflicted with Multiple Sclerosis
AUTHORS:
Rasam Hajiannasab
KEYWORDS:
Multiple Sclerosis, Chemokine, Cytokines, Real Time PCR
JOURNAL NAME:
International Journal of Clinical Medicine,
Vol.7 No.9,
September
13,
2016
ABSTRACT: MS is a chronic inflammatory disease of central nervous system in which T cells enter central nervous system and create an inflammatory cascade that leads to applying the other blood cells. Pre-inflammatory Cytokines are representative of the process of inflammatory diseases like MS. In addition, the increase of such cytokines has been observed in this kind of anomalies. On the other hand, helping T cells of 17 (TH17) contributes to the secretion of interleukin 17 that is a pre-inflammatory cytokine and an increase in TH17 cells is induced by interleukin 23 (IL23) which in turn causes absorption of neutrophils in the site of inflammation. Chemokine also has a determining function in the immune system including Ccl4 protein sit, the function of which is chemical absorption for calling natural fatal cells and monocytes as well as the other immune system cells; additionally, Ccl5 causes chemical absorption to absorb Eosinophil and Basophil and has an active role in applying Leukocytesin the site of inflammation. As a result, these 4 factors can hurt nervous cell through increasing its expression and calling leukocytes. The expression of these 4 genes, corticosteroid treatment and investigating its impact on changing the expression of such genes are the aims of this study. In this study 50 samples of blood of people suffer from multiple sclerosis (according to the diagnosis of specialist) (new case), 25 samples of blood of people suffer from MS (according to the diagnosis of specialist) using corticosteroid and 50 blood samples of healthy people without any symptoms were compared. To investigate the extent of expression of IL23, IL-17, CCL5 and CCL4 genes, first, the patients’ blood is taken, RNA was extracted and Real Time PCR was carried out. According to the results obtained from Real Time PCR of patients and healthy people it was specified that the amount of expression of chemokine and cytokines is increased in the people suffering from MS and has a direct relationship with MS. In comparing the two groups of patients (new case and people taking medicine) it was revealed that the amount of expression of IL17, IL23, CCL4 and CCL5 genes is decreased in people who take medicine. An increase in the expression of IL17, IL23, CCL4 and CCL5 genes is the symptom of MS disease and contributes to its creation and progression. The difference in the expression of chemokine and cytokine genes can be used as an identifying maker in this disease. On the other hand, corticosteroid medicine can have a determining role in the increase of expression as well as the destructive function of immune system.