TITLE:
Lysine Production of Microbacterium lacticum by Submerged Fermentation Using Various Hydrocarbon, Sugar and Nitrogen Sources
AUTHORS:
C. C. Ezemba, C. A. Ozokpo, V. N. Anakwenze, G. C. Anaukwu, C. M. Ogbukagu, C. C. Ekwealor, I. A. Ekwealor
KEYWORDS:
Lysine, Microorganism, Submerged Medium, Soil, Microbacterium lacticum
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.11,
September
7,
2016
ABSTRACT: Bacterial isolation from oil-contaminated and
uncontaminated soil was screened for hydrocarbon utilizer which was also
capable of producing lysine. Microbial production of lysine by Microbacterium lacticum was investigated
in submerged fermentations using various concentrations of hydrocarbon, sugar
sources and nitrogen. Of the nine sugar and five nitrogen sources tested,
glucose/ammonium sulphate proved optimum for lysine production. Effect of
varying concentration of carbon and nitrogen sources on lysine accumulation
showed that glucose (4%) ammonium sulphate (1%) respectively increased lysine
production. A gram positive rod bacterium identified as Microbacterium lacticum was identified. Optimizing the cultural
conditions of Microbacterium lacticum in submerged medium gave a methionine yield of 2.99 mg/ml lysine in the broth
culture after 96 h.