TITLE:
Combined Use of NMR, LC-ESI-MS and Antifungal Tests for Rapid Detection of Bioactive Lipopeptides Produced by Bacillus
AUTHORS:
Mara Silva, António Pereira, Dora Teixeira, António Candeias, Ana Teresa Caldeira
KEYWORDS:
Lipopeptides, Bacillus sp., Biodegradation/Biodeterioration, Natural Biocides, Nuclear Magnetic Resonance, Liquid Chromatography Coupled to Mass Spectrometry
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.10,
September
6,
2016
ABSTRACT: The science and technology interact with the art in
several ways. Biotechnological coupled with analytical approaches can play an
important role in protecting and preserving cultural heritage for future
generations. Many microorganisms influenced by environmental conditions are the
main responsible for biological contamination in built heritage. Biocides based
on chemical compounds have been used to mitigate this problem. Thus, it is
vitally important to develop proper remediation actions based on
environmentally innocuous alternative. Bacillus specie is emerging as an optimistic alternative for built heritage treatment
due to their capacity to produce secondary metabolites with antagonistic
activities against many fungal pathogens. Therefore, the intent of this work
was to access a rapid evaluation of antifungal potential of bioactive
metabolites produced by Bacillus strains and simultaneously their characterization using spectroscopic (NMR) and
chromatographic techniques (LC-ESI-MS). The high antifungal activity obtained for Bacillus sp. active compounds produced in this study confirms the
great potential to suppress biodeteriogenic fungi growth on historical
artworks. Additionally, the proposed methodology allowed to access bioactive
metabolites produced without need of the laborious total previous isolation and
could be used as a viable alternative to be employed for screening and
production of new green biocides.