TITLE:
Comparison of GeneXpert MTB/RIF Assay in Broncho Alveolar Lavage and Gastric Lavage Samples
AUTHORS:
Ravi Mahat, Nadeem Rizvi, Nausheen Saifullah, Madiha Tawfik, Ashok Kumar
KEYWORDS:
Mycobacterium tuberculosis Complex, Rifampicin Resistance, Bronchoscopy, TB Suspects
JOURNAL NAME:
Advances in Infectious Diseases,
Vol.6 No.3,
September
5,
2016
ABSTRACT: Objective: To compare the yield from
Gastric lavage (GL) and Broncho alveolar lavage (BAL) samples in adult patients
suspected case of Tuberculosis but not producing sputum. Methodology: 80 adults
with suspected case of tuberculosis but not producing sputum were recruited. 72
patients were then subjected to one gastric lavage followed by Broncho-alveolar
lavage in the same morning. The collected samples were subjected toGeneXpert
MTB/RIF assay. Result: Of the 72 patients samples, the mean age was 38.6 years.
41 (56.9%) were male and 31 (43.1%) were female. History of TB contact was
present in 25 (34.7%) patients. 37 (51.4%) patients had GeneXpert MTB/RIF
positive on BAL and/or GL samples. The GeneXpert MTB/RIF of BAL fluid was
positive on 35(48.6%), which was not significantly greater than that for
specimens from GL, which was 28(38.9%) (p > 0.05). In 26 (36.1%)
cases, GeneXpert MTB/RIF was positive in both BAL and GL samples. Conclusion:
This study showed the yield of GeneXpert MTB/RIF in GL was comparable to BAL to
detectMycobacterium tuberculosiscomplex. Patients who can’t
produce sputum, GL can be a good alternative to BAL to detect Mycobacterium
tuberculosiscomplex in resource poor areas and patients who do not
tolerate Bronchoscopy.