TITLE:
A Correlative Study between CT Perfusion Parameters and Angiogenesis in Rabbit VX2 Liver Tumors
AUTHORS:
Hao Xu, Xuli Min, Kang Liu, Lin Yang
KEYWORDS:
CT Perfusion Imaging, VX2 Liver Tumor, Vascular Endothelial Growth Factor, Hypoxia-Inducible Factor-1 Alpha, CD34 Molecule, Microvessel Density
JOURNAL NAME:
Open Journal of Medical Imaging,
Vol.6 No.3,
August
25,
2016
ABSTRACT: Objective: The purpose of this study was to
evaluate the correlation between CT perfusion parameters and the
hypoxia-inducible factor-1 alpha (HIF-1α), vascular en-dothelial growth factor
(VEGF), matrix metalloproteinase-2 (MMP-2) and microvessel density (MVD) marked
by CD34 molecular of rabbit VX2 liver tumors and to investigate the value of CT
perfusion imaging in evaluating tumor angiogenesis. Material and methods:
Twenty-four cases of rabbit VX2 liver tumor were performed by CT perfusion
scanning. Hepatic artery perfusion (HAP), portal vein perfusion (PVP), total hepatic
blood flow (THBF) and hepatic perfusion index (HPI) were measured by perfusion
software. HIF-1α, VEGF and MMP-2 expression and MVD were detected in the 24
rabbit VX2 liver tumor tissue samples using immunohistochemical method. The
correlation between the HIF-1α, VEGF, MMP-2 expression and MVD and CT perfusion
parameters were analyzed. Results: Correlation analysis revealed that the expression
of HIF-1α, MMP-2, MVD were positively related to the HAP, THBF, HPI (p 0.05); and correlation analysis
revealed that the expression of VEGF was positively related to the HAP, HPI (p
0.05). There was a
positive relationship between the expression of HIF-1α, VEGF, MMP-2 and MVD (p