TITLE:
The Structural Proteins of Bdellovibrio bacteriovorus Bacteriophage MAC-1
AUTHORS:
Rajinder S. Ranu, Renee Gallegos, Mary Althauser, Lisa Wolfe
KEYWORDS:
Bdellovibrio bacteriovorus Bacteriophage, MAC-1 Phage Structural Proteins, MAC-1 Genome, MAC-1 Genes (ORFs)
JOURNAL NAME:
World Journal of Engineering and Technology,
Vol.4 No.3B,
August
25,
2016
ABSTRACT: In
the present investigation the structural proteins associated with MAC-1
bacteriophage have been characterized using sodium dodecyl
sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); tandem mass spectrometry
of protein bands from SDS-PAGE gel; from the open reading frames (ORFs) deduced
from MAC-1 genome sequence and amino acid sequence homology searches from the
Uniprot database (up000002418). Results have led to the identification of at
least three structural proteins associated with MAC-1 phage genome. They are:
capsid protein (~55,000-daltons); spike protein (~22,000-daltons) and a low
molecular weight DNA binding protein (~4000-dal- tons). In addition, two other
minor proteins were tentatively identified as replicative and scaffold proteins
based on two to three unique peptides from mass spectrometry data. However,
other proteins coded (ORFs) by phage genome remain to be identified.