TITLE:
Exposure of Common Bean Seeds to Liquid Nitrogen Modifies Mineral Composition of Young Plantlet Leaves
AUTHORS:
Inaudis Cejas, Annekathrin Rumlow, Ariel Turcios, Florent Engelmann, Marcos Edel Martínez, Lourdes Yabor, Jutta Papenbrock, José Carlos Lorenzo
KEYWORDS:
Common Bean, Cryopreservation, Cryostorage
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.12,
August
23,
2016
ABSTRACT: Many publications
describe cryopreservation techniques but only a few studies have focused on the
biochemical and physiological changes occurring in plants regenerated from
seeds exposed to liquid nitrogen. This paper aims at describing the effect of
common bean seed cryostorage on mineral nutrition of young plantlets. The
following elements were measured on leaves of 10-day-old plantlets from
non-cryopreserved and cryopreserved seeds: Al, B, Ba, Ca, Cd, Co, Cr, Cu, Fe,
K, Mg, Mn, Na, Ni, P, S, Se, Sr and Zn. At 10 days after sowing, both
treatments (control and cryopreserved seeds) showed 100% seed germination
without any visual phenotypic difference. However, contents of several elements
in the leaves were different. Exposure of seeds to liquid nitrogen decreased
Cu, Cd and Na uptake and increased absorption of B and Al. Further studies are
required to understand the mechanisms underlying the relationship between seed
exposure to liquid nitrogen and mineral nutrition during the early stages of
plantlet growth.