TITLE:
Quantitative and Qualitative Study of Phenolic Compounds Involved in Germination Inhibition of Wheat under Water Deficit
AUTHORS:
Abdelghani Chakhchar, Salama Aissam, Cherkaoui El Modafar
KEYWORDS:
Triticum aestivum L., Germination, Water Deficit, Polyphenols, Ferulic Acid
JOURNAL NAME:
Technology and Investment,
Vol.7 No.3,
August
19,
2016
ABSTRACT: In the aim to study the contribution of phenolic compounds in the inhibition of seeds germination of wheat (Triticum aestivum L.) under water deficit, we have forwarded quantitatively and qualitatively the change of the phenolic compounds levels in the seeds of two varieties (Achtar and Tigre) submitted to water stress induced by PEG-6000 during the germination stage. The results revealed significant differences between the stress intensity and duration and the polyphenol content. Some germination traits were significantly correlated with water stress and polyphenols in the both varieties studied. The polyphenol content in Tigre was much higher than those noted in Achtar. Analysis of phenolic extracts by HPLC showed the dominance of hydroxycinnamic derivatives (97.2% and 94.5% in Tigre and Achtar, respectively). Regarding phenolic acid, the ferulic acid was the most dominant, and seemed to be related mainly to the inhibition of germination. Quantitatively, the Achtar variety was characterized by the presence of acid sinapic which was absent in Tigre variety. Furthermore, the water deficit appeared to stimulate the induction of salicylic acid accumulation in both varieties.