TITLE:
Effects of Garcinia kola and Azadirachta indica Seeds in the Inhibition of Aspergillus flavus and Aspergillus parasiticus Isolated from Zea mays L. Awka, Nigeria
AUTHORS:
A. N. Achugbu, J. E. Amadi, C. V. Ilodibia, M. N. Ikegbunam
KEYWORDS:
Garcinia kola, Azadirachta indica, Inhibition, Aspergillus Species, Zea mays
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.11,
August
19,
2016
ABSTRACT: Crude extracts of neem and
bitter kola seeds were evaluated for inhibition of the growth of aflatoxin-producing
fungi (Aspergillus flavus and A. parasiticus) in Zea mays L. Different concentrations of the methanolic and ethanolic extracts of the test
plants were tested individually and in combination against A. flavus and A. parasiticus.
Inhibition of A. flavus using methanolic extracts of G. kola, neem and combination of (G. kola and neem) seeds revealed that inhibition was highest in G. kola (77.5) at 10% concentration, and
lowest in neem (35.1) at 2.5% concentration. Statistical analysis showed a significant
difference at 5%. Inhibition of A. flavus using ethanolic extracts of G. kola, neem and combination of G. kola and neem seeds revealed that inhibition
was highest in the combination of (80.4) at 10% concentration and lowest in G. kola (31.4) at 2.5% concentration. Using
methanolic extracts of G. kola, neem and
combination against A. parasiticus showed that inhibition was highest
in G. kola and combination of seeds (54.8)
at 10% concentration and lowest in neem seeds (30.5) at 5.0% concentration. The
percentage inhibition of A. parasiticus using ethanolic extracts of G. kola, neem and combination revealed that
inhibition was highest in G. kola (79.5) at 10% concentration and lowest in the combination
of G. kola and neem seeds (26.6) at 2.5% concentration. These results will be important in planning a management strategy
against A. flavus and A. parasiticus and other fungi associated with
spoilage of stored products.