TITLE:
Construction and Analysis of the Immune Effects of a Streptococcus agalactiae Surface Protein ScpB Vaccine Encapsulated with Polylactic-Co-Glycolic Acid (PLGA)
AUTHORS:
Xiaoli Ke, Qingyong Li, Xiaotian Li, Zhigang Liu, Maixin Lu, Hong Yang
KEYWORDS:
Tilapia, Streptococcus agalactiae, C5a Peptidase, Immunogenicity
JOURNAL NAME:
Open Access Library Journal,
Vol.3 No.8,
August
12,
2016
ABSTRACT:
In order to find an effective immune preparation to control
tilapia streptococcus disease, the Streptococcus agalactiae surface protein serine protease
C5a peptidase (ScpB) was cloned and the recombinant protein was encapsulated in
poly (lactide-co-glycolic acid) (PLGA) microspheres, which were comprised of biodegradable
materials. The ScpB-PLGA vaccine was then administered to the tilapia intraperitoneally
at different concentrations, with PBS used as a control, and the relative percent
survival (RPS) of each group was calculated. Serum lysozyme and superoxide dismutase
(SOD) activity levels and antibody levels (OD450nm) were tested weekly
for the duration of the experiment. The results showed that the ScpB loading rate
in the PLGA microspheres was 2.55% and the encapsulation efficiency reached 48.76%.
The RPS ranged from 66.80% to 87.66%, with the highest RPS noted in group P1 (1
μg/g). The serum lysozyme, SOD and antibody (IgM) levels were significantly higher
in the vaccinated fish relative to the control groups (P