TITLE:
A Possible Mechanism of DNA to DNA Transcription in Eukaryotic Cells: Endonuclease Dependent Transcript Cutout
AUTHORS:
Gao-De Li
KEYWORDS:
Plasmodium falciparum, Endonuclease Dependent Transcript Cutout, Mechanism, Eukaryotic Cell, Chromatin Conformation, Dynamic Genome Architecture, DNA to DNA Transcription, Nicking Endonuclease, ssDNA Transcript, Cell Cycle Progression, Chloroquine
JOURNAL NAME:
Open Access Library Journal,
Vol.3 No.6,
June
17,
2016
ABSTRACT: We previously proposed the existence of DNA to DNA transcription in
eukaryotic cells, but the mechanism by which single-stranded DNA (ssDNA)
transcript is produced and released from the genome remains unknown. We once
speculated that the mechanism of DNA to DNA transcription might be similar to
that of DNA to RNA transcription, but now we propose that endonuclease dependent
transcript cutout may be a possible mechanism of DNA to DNA transcription, in
which a copy of ssDNA fragment (transcript) between two nicks produced by nicking
endonuclease is released from double-stranded DNA (dsDNA) region in the genome
by an unknown ssDNA fragment releasing enzyme. The gap in the dsDNA will be
filled through DNA repair mechanism. Occasionally, multiple copies of ssDNA
transcripts could be produced through multiple rounds of cutout-repair-cutout
cycle.