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Cho, H.S., Mason, K., Ramyar, K.X., Stanley, A.M., Gabelli, S.B., Denney, D.W., et al. (2003) Structure of the extracellular region of HER2 alone and in complex with the Herceptin Fab. Nature, 421, 756-760.

has been cited by the following article:

• TITLE: Enhanced immuno-detection of shed extracellular domain of HER-2/neu

  AUTHORS: Sinang Chourb, Brian C. Mackness, Leslie R. Farris, Melisenda J. McDonald

  KEYWORDS: HER-2/neu; Shed Extracellular Domain; C-erbB2; Breast Cancer Marker; ELISA; Fluorescent immunoassay; ALYGNSA

  JOURNAL NAME: Health, Vol.1 No.4, December 31, 2009

  ABSTRACT: HER-2/neu oncogene is over-expressed and amplified in patients associated with metastatic breast cancer. An increased level (>15 ng/mL) in the shed extracellular domain (sECD-HER 2/neu) is indicative of the potential presence and as-sociated progression of this disease. A fluo-rescent ELISA incorporating the newly devel-oped ALYGNSA antibody-orientation system revealed a 10-fold increase in sensitivity (≤0.63 ng/mL) of sECD-HER 2/neu when compared to a control standard ELISA kit (≤7.5 ng/mL). This enhanced mode of detection has the potential to not only address breast and other cancers per se but also permit an in depth evaluation of “shed extracellular domains”, in general, and the role of these “proteolytic derived factors” in physiological signalling at normal levels.