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Suri, S., Ruan, G., Winter, J. and Schmidth, C. (2013) Microparticles and Nanoparticles. In: Ratner, B., Hoffman, A., Schoen, F. and Lemons, J., Eds., Biomaterials Science: An Introduction to Materials in Medicine, Elsevier, San Diego, 360-388.

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• TITLE: Genipin Cross-Linked Polyvinyl Alcohol-Gelatin Hydrogel for Bone Regeneration

  AUTHORS: Thi-Hiep Nguyen, Reiza Ventura, Young-Ki Min, Byong-Taek Lee

  KEYWORDS: PVA-Gelatin, PVA, Gelatin, Genipin, Hydrogel, Bone Regeneration

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.9 No.9, August 2, 2016

  ABSTRACT: Polyvinyl alcohol gelatin hydrogels were fabricated using genipin as a crosslinking agent for bone regeneration application. Optimized formulation of PVA-GE hydrogel was fabricated using genipin as crosslinking agent. Characterizations such as FTIR, morphology, porosity, pore size, degradation and swelling rate were investigated. Bone regeneration potential of optimized genipin cross-linked polyvinyl alcohol-gelatin (PVA20) hydrogels was assessed by implanting in rabbit’s femur defect for 1, 5 and 15 weeks period. Results showed interconnected porosity as observed in scanning electron microscopy and successful crosslinking as confirmed by FTIR analysis. Increased porosity (92% ± 2.46%) and pore size distribution (100 - 200 μm) were also observed as well as decrease in swelling rate (426% ± 10.50%). Bone formation was evident in micro-CT after 5 and 15 days of in vivo implantation period. Micro-CT analysis showed 32.67% increased bone formation of PVA-GE hydrogel defect compared with negative control after 15 weeks of in-vivo implantation. Histological analyses showed no inflammatory reaction post implantation and increase in cell matrix formation after 5 and 15 weeks. The combined physical and chemical method of crosslinking promises improved mechanical properties of PVA-GE hydrogel making it a potential scaffold for bone tissue engineering applications.