TITLE:
Differential Anti-Inflammatory Effects of Three Purified Omega Unsaturated Fatty Acids on Collagen-Induced Arthritis in Mouse
AUTHORS:
Pamela Izaret Pérez-Martínez, Víctor Gabriel Hernández, Oscar Rodríguez-Espinosa, Patricia Arce-Paredes, Oscar Rojas-Espinosa
KEYWORDS:
Collagen, CIA, DBA-1 Mice, Omega-UFAs, Dexamethasone, Anti-Inflammatory
JOURNAL NAME:
Modern Research in Inflammation,
Vol.5 No.3,
August
2,
2016
ABSTRACT: Background: The
Mediterranean Diet (MD) has been linked to a reduced risk of developing
degenerative diseases, including atherosclerosis, heart stroke, diabetes,
arthritis and cancer. However, only a few scientific investigations have
attempted to validate this impression. The ingredients of the MD include
significant amounts of omega (ω3,ω6,
andω9) unsaturated fatty
acids (UFAs). A few studies of these UFAs in the prevention or treatment of
arthritis have yielded controversial results, but a general belief regarding
their beneficial effects has prevailed. Objective: To investigate the effects
of three relevant UFAs, namely Docosahexaenoic Acid (DHA), Arachidonic Acid
(AA), and Oleic Acid (OA) (ω3,ω6,
andω9, respectively), in
the development of arthritis using a murine model of Collagen-Induced Arthritis
(CIA). Methods: DBA-1 mice were immunized with chicken collagen type II (CII)
and were subsequently treated withω-UFAs
for 53 days. Dexamethasone (DEXA) was used as a positive anti-inflammatory
agent. The effect of the treatments was evaluated through several parameters:
inflammation indices, antibody levels, cell prolifera- tion, and
histopathological findings. Results and Conclusion: The anti-inflammatory
effect of the tested substances was inversely correlated with the
histopathological findings: a greater anti- inflammatory effect was
associated with less articular damage. Oleic acid (ω9) was the most
efficient anti-inflammatory UFA, followed by DHA and then AA. DEXA completely
inhibited the development of arthritis, whereas the untreated CII-immunized
mice developed the most severe articular damage. DBA-1 mice with CII-induced
arthritis constitute an adequate model for the study of arthritis and its
treatment.