TITLE:
Specifics of Platelet Hemostasis in Children with Chronic Diseases of Lungs
AUTHORS:
Olga B. Gordeeva, Victoria V. Botvinieva, Olga I. Simonova, Leyla S. Namazova-Baranova, Yulia V. Gorinova, Nato D. Vashakmadze, Anna K. Gevorkyan
KEYWORDS:
Platelet Aggregation Function, Cystic Fibrosis, Inflammation, Children
JOURNAL NAME:
Open Access Library Journal,
Vol.3 No.4,
April
28,
2016
ABSTRACT: Study of platelet aggregation function can help early diagnostics of microvasculature’s
disorders. Purpose of the study is evaluation of changes of platenet aggregation
activity in children with chronic inflammation. Materials and methods: 85 children
examined, of whom 45 children with cystic fibrosis (mixed form) aged 8 months to
17 years. The platelet aggregation in children’s whole blood was studied using aggregometer
“Multiplate” (VerumDiagnostica, Germany). The following agents were used as inducer
of aggregation: adenosine diphosphoric acid solution (ADP), soluble Thrombin Receptor
Activator for Peptide 6 (TRAP-test) and arachidonic acid (ASPI-test). C-reactive
protein (SRP) in the blood serum was measured by turbidimetric method using biochemical
analyzer UniCelDxC 600 (BD, USA). Immunochemiluminescent method was used to determine
the level of the main pro-inflammatory cytokine IL-6 (analyzer Accesses 2, BD, USA).
Results: changes in the aggregation of platelets can be expressed by platelet activation
followed by hyper-aggregation and thrombi formation on the one hand, and disorder
of arachidonic acid and ADP metabolism on the other hand that leads to the development
of hemorrhagic complications. A necessary condition of the therapy is a presence
of antiplatelets and calcic drugs, which help to warn complications in a form of
thrombosis and hemorrhages.