TITLE:
Effect of Chitosaccharides in Nodulation and Growth in Vitro of Inoculated Soybean
AUTHORS:
Daimy Costales, Alejandro Bernardo Falcón, María Caridad Nápoles, Julien de Winter, Pascal Gerbaux, Robertus Cornelis Adrianus Onderwater, Ruddy Wattiez, Juan Carlos Cabrera
KEYWORDS:
Chitosan, Glycine max, Bradyrhizobium, Antimicrobial
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.9,
July
21,
2016
ABSTRACT: The influence of
chitosaccharides on the symbiotic interaction between Bradyrhizobium and soybean was examined. The results show that
chitosaccharides either positively or negatively affect soybean nodulation or
plant growth depending on their molecular weight, concentration and the
application methods. When directly added to the in vitro culture media, chitosan of high molecular weight inhibit Bradyrhizobium viability in a dose
dependent manner while chitooligosaccharides reduce slightly the bacteria
viability only at concentration equal or higher than 50 mg·L-1. Chitooligosaccharides
significantly enhance nodule formation and dry mass in soybean roots at doses
between 10 and 100 mg·L-1. Both types of chitosaccharides,
at the highest doses (>500 mg·L-1), negatively affect plant
height and root size, whereas medium doses (50 to 100 mg·L-1) increase slightly leave
number. Under field conditions, foliar application of both chitosaccharides
enhances growth and nodulation of soybean plants. Nevertheless, using this
application method, chitosan remains more effective than chitooligosaccharides.