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Cole, J.R., Wang, Q., Fish, J.A., Chai, B., McGarrell, D.M., Sun, Y., Brown, C.T., Porras-Alfaro, A., Kuske, C.R. and Tiedje, J.M. (2014) Ribosomal Database Project: Data and Tools for High Throughput rRNA Analysis. Nucleic Acids Research, 42, D633-D642.
http://dx.doi.org/10.1093/nar/gkt1244

has been cited by the following article:

• TITLE: Temperature Gradient Gel Electrophoresis as a Valuable Accessory Tool for Assessment of Dysbiosis in Crohn’s Disease

  AUTHORS: Vanessa Rafaela de Carvalho, Rogeria Keller, Ana Carolina da Silva Santos, Josias Rodrigues

  KEYWORDS: Dysbiosis, Proteobacteria, Escherichia coli, Crohn’s Disease

  JOURNAL NAME: Advances in Microbiology, Vol.6 No.8, July 20, 2016

  ABSTRACT: Escherichia coli and other Proteobacteria are augmented and several other bacteria are diminished in Crohn’s (CD) disease patients’ intestine. This imbalance in bacterial species composition—termed dysbiosis—seems to be determinant of CD manifestation. Since a great part of intestinal bacteria are not cultivable, detection of CD dysbiosis is accomplished by molecular tools, involving sequences analysis of the 16SrRNA gene (16SrDNA) present in the patient’s clinical samples, which can be done by sequencing or electrophoresis in denaturing gels of 16SrDNA amplicons. By analyzing, by temperature gradient gel electrophoresis (TGGE) and next generation sequencing of 16SV6-V8rDNA amplicons present in gram negative cultures from four distinct clinical samples of a control subject and a CD patient, this study demonstrates that both techniques were able to detect E. coli overgrowth and reduction in species richness in CD and that TGGE can discriminate sequences collectively labeled as “unclassified” in 16SrDNA databases. Although TGGE per se does not identify the sequences, the discriminatory power that it confers represents valuable accessory information to next generation DNA sequencing (NGS), and as such must be used as a NGS complementary tool.