TITLE:
Effect of Morphological and Physiological Development on the Acclimatization of in Vitro Plants of Bambusa vulgaris Schrad ex Wendl in Liquid Culture Medium
AUTHORS:
Yudith García-Ramírez, Mallelyn González-González, Marisol Freire-Seijo, Mariana La O-Cárdenas, Miladys León-Quintana, Berkis Roque-Morales, Eilyn Mena-Méndez, Miladys Francisca Mendoza-Rodríguez, Leonardo Rivero-Quintana
KEYWORDS:
Bamboo, In Vitro Multiplication, Ex Vitro Survive
JOURNAL NAME:
Open Access Library Journal,
Vol.2 No.9,
September
14,
2015
ABSTRACT: Bambusa
vulgaris Schrad. ex Wendl (B. vulgaris)
is a species of great interest because it produces the most important
non-timber forest products of worldwide. It has become an integral component
in social forestry programs. This study aimed to determine the effect of
morphological and physiological development on the acclimatization of in vitro plants of B. vulgaris. Morphological and physiological parameters of plants
subjected to in vitro conditions were
evaluated. Our results showed that the number shoots per explant of B. vulgaris increased when 6.0 μM of
6-benzylade-nine (BA) was incorporated into the culture medium. After six
subcultures, clumps of 3 - 6 axes were obtained, and their division in groups
of three axes allowed multiplication of the plants. During the acclimatization
phase to ex vitro conditions, the in vitro plants showed 75% survival.
Results showed that greater development of the root system, height and number
of leaves per plants was observed with height larger of 3.0 cm. The leaf area,
water content, fresh and dry weight
increased with height larger of 3.0 cm, but all parameters were decreased with
height (1.0 - 3.0 cm). The correlation between net assimilation rate and leaf
area index was significantly positive in
vitro plants with height larger of 3.0 cm. The increase of plants of B. vulgaris in liquid culture medium
offers new prospects for increasing the commercial production of this species,
and for studies that could elucidate morphological and physiological responses
in plants of bamboo.