TITLE:
Comparison of Two Parasitological Tests and Post Mortem Examination to Detect Prevalence of Bovine Fasciolosis at Ambo Town Municipal Abattoir, West Shewa Zone, Ethiopia
AUTHORS:
Diriba Taddese Legesse, Feyera Gemeda
KEYWORDS:
Ambo, Bovine Fasciolosis, Direct Smear, Sedimentation, Prevalence, Post Mortem Examination
JOURNAL NAME:
Open Access Library Journal,
Vol.2 No.8,
August
21,
2015
ABSTRACT: A cross sectional study was conducted from
March 5, 2013 to June 28, 2013 in Ambo town municipal abattoir to assess
prevalence of bovine fasciolosis and compare tests used for bovine fasciolosis.
Direct smear, sedimentation and post mortem examination were the tests used for
comparison. Post mortem examination was considered as gold standard test. Data
were collected by using primary data at the abattoir; Kappa (K) and percentage
were utilized to assess agreement of tests and prevalence, respectively. A
total of 60 fecal samples were collected from cattle brought to the abattoir
for direct smear and sedimentation techniques. Adult Fasciola were detected from slaughtered
cattle at the abattoir. An overall prevalence of 21.66% (13 of 60), 18.33% (11
of 60) and 13.3% (8 of 60) were obtained by post mortem examination,
sedimentation and direct smear techniques, respectively. At species level,
69.23% (9 of 13 positive cases) Fasciola
hepatica and 84.62% (11 of 13 positive cases) Fasciola gigantica prevalence were observed. From 13 positive
samples, 7 (53.85%) were mixed. Sedimentation showed higher agreement with post
mortem examination (K = 0.896) than direct smear with post mortem examination
(K = 0.715). The specificity of direct smear, sedimentation and post mortem
examination were 61.53%, 84.6% and 100%, respectively. In conclusion, presence
high prevalence of mixed infestation indicated that presence of suitable
ecology for both Fasciola species and
sedimentation were considered as better test than direct smear. Therefore,
control strategies and utilization of sedimentation technique to detect Fasciola egg for samples collected from
live animals were recommended.