TITLE:
Comparative Optimized Protocols of DNA Extraction and Purification Using FTA PlantSaver Card and DNAzol Methods for Eggplant (Solanum Species) Studies in North Central Nigeria
AUTHORS:
Celestine Uzoma Aguoru, Lucky O. Omoigui, Joseph Olalekan Olasan
KEYWORDS:
Optimization, DNA Extraction, FTA PlantSaver Card, DNAzol, Protocols, Eggplant, Solanum Species
JOURNAL NAME:
Open Access Library Journal,
Vol.2 No.4,
April
2,
2015
ABSTRACT: Two methods of DNA extraction and purification in eggplant molecular research were investigated. FTA PlantSaver card and DNAzol were optimized, simplified and presented. The aim was to discard the rigorous protocols and help save energy, resources and time wasted in DNA extraction during molecular research in eggplant. DNA was extracted from fresh leaf of 18-day-old eggplant seedlings. Quality of genomic DNA extracts was verified on 1% agarose gel electrophoresis and viewed on a UV-transilluminator. DNA extracted from nine (9) different accessions was PCR amplified using nine (9) RAPD primers. Results indicated that both methods were effective in extracting and purifying sufficient quantity of DNA under different requirements. DNAzol method required the use of more leaf samples to extract sufficient quantity of DNA which in turn required that more seeds should be sown per accession. However, sufficient quantity of DNA could be extracted from even a single leaf sample using the FTA card method which may be preferred in a situation of low seed viability. Therefore, FTA card method is recommended for timely and quality DNA extractions and amplification using simple protocols outlined in this article.