TITLE:
Protective Effect of bFGF on Bone Marrow Mesenchymal Stem Cell against Hydrogen Peroxide-Induced Apoptosis
AUTHORS:
Xiaoqing Gao, Guangqiang Hu, Guangbi Fan, Zhiqiang Mei, Li Deng, Jie Du
KEYWORDS:
Basic Fibroblast Growth Factor, Bone Marrow Mesenchymal Stem Cells, Hydrogen Peroxide, Oxidative Stress, Apoptosis, Mechanism
JOURNAL NAME:
Open Access Library Journal,
Vol.3 No.2,
February
18,
2016
ABSTRACT: Bone marrow mesenchymal stem cells
(BMSCs) transplantation has emerged as a promising treatment for various central
nervous system diseases. However, oxidative stress results in poor survival of transplanted
BMSCs in injury central nervous system (CNS). Basic fibroblast growth factor (bFGF)
has been reported to have anti-apoptotic properties in serial of cells in oxidative
stress. This study was designed to investigate protective effect and potential mechanisms
of bFGF against hydrogen peroxide (H2O2)-induced apoptosis
of BMSCs. BMSCs were pretreated with 20 ng/ml of bFGF for 30 minutes, followed by
exposure to 500 μM H2O2 and bFGF together for 1 hour. bFGF
treatment significantly attenuated H2O2-induced cytotoxicity
and apoptosis of BMSCs. Moreover, bFGF effectively decreased the levels of TNF-α and IL-6 and increased expression ratio
of Bcl-2/Bax in H2O2-induced BMSCs. The findings suggest that
bFGF is likely to be an effectively protective agent against oxidative stress-induced
apoptosis for BMSCs.