TITLE:
Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B
AUTHORS:
Erick Ntambwe Kamangu, Adawaye Chatte, Raphael Boreux, Fabrice Susin, Richard Lunganza Kalala, Georges Lelo Mvumbi, Patrick De Mol, Marie-Pierre Hayette, Dolores Vaira
KEYWORDS:
COBAS AmpliPrep/TaqMan, In-House PCR Assays, HIV-1 Non-B
JOURNAL NAME:
Open Access Library Journal,
Vol.2 No.3,
March
20,
2015
ABSTRACT: Context: The in-house techniques or experimental methods are increasingly
recommended for their low-cost reagents for the determination of the Viral Load
(VL) in resource-limited settings. The objective of this study was to compare
the determination of VL from HIV-1 non-B samples by an in-house technique with
the COBAS AmpliPrep/TaqMan version 2.0. Method: In this cross-sectional
study, 39 plasma samples from patients infected with HIV type 1 non-B from N’Djamena
and Kinshasa were used to determine the VL using the two techniques. Results: The
mean values of VL are respectively 4.68 ±
1.26 and 4.58 ± 1.33 log10 RNA copies/ml for the COBAS AmpliPrep/TaqMan
assays and the in-house assays. A good correlation (Spearman Correlation) was
obtained, with a coefficient (R2) of 0.9452. Conclusion: This study
demonstrates that there is no significant difference between the results of VL
determined by the COBAS AmpliPrep/TaqMan assays and the in-house assays used.