TITLE:
In Vitro Micropropagation of Himalayan Weeping Bamboo, Drepanostachyum falcatum
AUTHORS:
Himanshu Saini, Inder Dev Arya, Sarita Arya, Reetu Sharma
KEYWORDS:
In Vitro, Drepanostachyum falcatum, Himalayan Weeping Bamboo, Plant Growth Hormone, Micropropagation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.9,
July
8,
2016
ABSTRACT: Plant growth hormone
BAP (benzyl amino purine), KIN (kinetin), NAA (1-naphthalene acetic acid) and
IBA (indole-3 butyric acid) effect was studied on in vitro multiplication of shoots and rooting of Drepanostachyum falcatum. In vitro micropropagation of himalayan
weeping bamboo is explained by in vitro shoot induction and proliferation. Excised explant with axillary bud is surface
sterilized with 0.1% HgCl2 for 10 - 12 minutes, cleaned with 90%
ethanol and inoculated on liquid Murashige and Skoog (MS) culture medium
supplemented with different concentrations of BAP/ KIN. Effect of BAP/KIN on
shoot induction is with different rate and number of shoots produced by
explants with axillary bud cultured on MS media supplemented with 0.0 mg/L
BAP/KIN - 5.5 mg/L BAP/KIN. Shoot multiplication with highest rate is achieved
on MS medium supplemented with 3.5 mg/L BAP after 4th sub-culturing.
The most effective with highest rate and number of root induction combination is
6.5 mg/L IBA after 5 weeks. The roots produced by 6.5 mg/L IBA is best compared
with other combination of auxin NAA (1-naphthalene acetic acid).