TITLE:
Potential Antimicrobial Effects of Gatifloxacin on Periodontopathic Bacteria in Vitro
AUTHORS:
Tadashi Miura, Koji Tanabe, Eri Tsukagoshi, Koichi Kida, Yasuhiko Shizawa, Nahoko Miyake, Masataka Kasahara, Masao Yoshinari
KEYWORDS:
Local Administration, Peri-Implantitis, Periodontopathic Bacteria, Antimicrobial Activity, Gatifloxacin
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.9 No.7,
June
21,
2016
ABSTRACT: The aim of this study was to identify the potential antibacterial effects of gatifloxacin on periodontal pathogens including Aggregatibacter actinomycetemcomitans, Porphyromonas gingi-valis, and Prevotella intermedia. The minimum inhibitory concentrations (MIC) of gatifloxacin and its bactericidal effects were investigated. Gatifloxacin inhibited the growth of all three kinds of periodontopathic bacteria tested in broth. The MIC value of 2.5 nM was found to be the most effective in inhibiting A. actinomycetemcomitans. An adenosine triphosphate biolumi-nescence assay revealed that gatifloxacin exhibited bactericidal effects on the tested bacteria in a time-dependent manner. The safety of gatifloxacin in mammalian cells was evaluated by assessing the viability of normal human dermal fibroblast (NHDF) cells treated with gatifloxacin. Almost all NHDF cells survived after 2-d culture, while 81% of the cells survived after 4-d culture when treated with 1.0 × 103 nM gatifloxacin. These results indicate that gatifloxacin is a possible drug for local administration to prevent periodontal infection.