TITLE:
Effect of Hoechunyangkyeok-San Extract on Melanogenesis
AUTHORS:
Mi Jin Kim, Taek Kyu Jung, Hyun-Chul Park, Kyung-Sup Yoon
KEYWORDS:
Hoechunyangkyeok-San (Formula Extract Containing Forsythia viridissima Fruit, Platycodon grandiflorum Root, Glycyrrhiza glabra Root, Paeonia japonica Root, Angelica gigas Root, Scutellaria baicalensis Root, Gardenia jasminoides Fruit, Coptis chinensis Root, Mentha arvensis Leaf, Rehmannia glutinosa Root, Poncirus trifoliate Fruit), Melanogenesis, Melanosome Transport, Melanin, Whitening
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.6 No.3,
May
26,
2016
ABSTRACT: Forsythia fructus has been shown to have antioxidative,
anti-inflammatory, antibacterial, anti-aging and whitening effects. Hoechunyangkyeok-san
(Forsythia viridissima-prescription)
is a traditional herbal medicine, which has been clinically used for treating
febrile and inflammatory disorders. This work was carried out to investigate
the skin whitening effects of Forsythia
viridissima-prescription extract (a hydrolyzed extract of
Hoechunyangkyeok-san: SID White HYC) on skin. The effects of SID White HYC were
assessed the melanin contents in B161 melanoma cells and the pigmented
equivalent with HMB45 and Fontana Masson staining in 3D skin model. Then, we
examined the expression of major pigment enzymes regulating melanin synthesis
and melanosome transport related proteins in B16F1 cells. SID White HYC
significantly inhibited the melanin synthesis (56.7% and 30.6% inhibition at
100 μg/mL, intracellular and secreted, respectively) in B16F1 cells and 3D skin
model. In addition, western blotting analysis showed that SID White HYC reduced
the expression of melanin synthesis and melanosome transport related proteins
in B16F1 cells. In clinical trials, the cream containing 0.05% SID White HYC
showed skin depigmentation effect without any irritation. These results suggest
that SID White HYC may be useful inhibition of melanogenesis and melanosome
transport. Therefore, SID White HYC may have potential as a skin-whitening
ingredient in cosmetics.