TITLE:
Antimutagenic and DNA Damage Protective Activities of a Grape Extract from Vitis vinifera
AUTHORS:
Zuzana Rybková, Kateřina Malachová, Jiří Červeň, Hana Sezimová, Petr Pečinka
KEYWORDS:
Ames Test, Antimutagenicity, Free Radical Scavenging, DNA Strand Scission, Vitis vinifera
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.4 No.6,
May
24,
2016
ABSTRACT: Antimutagenic and DNA protective effect of an extract VinOserae from Vitis vinifera grapes on oxidative DNA damage was investigated. The extract’s ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BHP) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His? TA102 strain. Inhibition values of 44.2% and 67.0% were detected for t-BHP and H2O2, respectively. A protective ability of the extract against DNA strand scission induced by hydroxyl radicals was studied with plasmid pBluescript II SK(-). The analysis of DNA strand breaks in plasmid DNA showed a significant inhibition of DNA damage.