TITLE:
Wavelength Selection for Near-Infrared Spectroscopic Analysis of Teicoplanin
AUTHORS:
Jing Zhang, Tian Ai, Jiemei Chen, Tao Pan
KEYWORDS:
Teicoplanin, Near-Infrared Spectroscopy, Equidistant Combination Partial Least Squares, Equivalence Model Set, The Simplest Equivalent Model
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.7 No.5,
May
19,
2016
ABSTRACT: Teicoplanin (TCP) is
a multiple drug-resistant lipoglycopeptide antibiotic produced by fermenting Actinoplanes teichomyceticus. In this study, a mixture of TCP with the Tris-HCl buffer
(TCP-Tris- HCl) was used to simulate TCP fermentation
broth. The reagent-free, rapid and simultaneous quantitative analysis models
for TCP and Tris in the TCP-Tris-HCl mixtures were established by near-infrared
(NIR) spectroscopy. The equidistant combination partial least squares (EC-PLS) method
and the equivalent model sets were proposed, the simplest equivalent model with
the smallest number of wavelengths were further selected. The initial wavelength,
number of wavelengths, number of wavelength gaps, number of PLS factors were 1520
nm, 28, 5, 5 for TCP and 1084 nm, 13, 6, 4 for Tris, respectively. Compared with
the optimal EC-PLS models, the simplest equivalent models adopted fewer wavelengths.
Thus, the redundant wavelengths were removed, the models were further simplified. The root-mean-square errors (SEP) and correlation
coefficients (RP) for prediction were 0.043 mg·mL-1 and 0.9998
for TCP, and 0.222 mg·mL-1 and
0.9989 for Tris, respectively. The results indicate that NIR method can be applied
to highly accurate quantitative analysis for TCP and provide valuable references
for further application to TCP fermentation broth.