TITLE:
Bioactive Compounds Produced by Streptomyces sp. Isolate UPMRS4 and Antifungal Activity against Pyricularia oryzae
AUTHORS:
Hayman Kakakhan Awla, Jugah Kadir, Radziah Othman, Tavga Sulaiman Rashid, Mui-Yun Wong
KEYWORDS:
GCMS, LCMS/MS, Antifungal, Streptomyces sp. and Rice Blast
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.7,
May
18,
2016
ABSTRACT: A Streptomyces isolate having
antifungal activity against Pyricularia oryzae, the causal agent of rice blast disease, was isolated from soil
collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia.The aim of the study was
to determine the antifungal activity of Streptomyces sp. isolate UPMRS4
extracts against P. oryzae and to identify bioactive antifungal
compounds produced by UPMRS4. Various solvents were used for extraction of
antifungal compounds and well diffusion method was used to determine the
antifungal activity of the extracts. The ethyl acetate extract demonstrated the
highest activity against mycelial growth of P.
oryzae, with an effective inhibitory concentration (EIC) of 1.562 μg/ml
significantly higher compared to that of chloroform, diethyl ether, methanol,
acetone, ethanol and water. Based on GC-MS and LC-MS/MSanalyses,compounds
with antifungal activity were detected such as (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl); Pyrrolo[1,2-a]
pyrazine-1,4-dione, hexahydro-3-(phenylmethyl); ergotamine; amicomacin; fungichromin;
rapamycin and N-Acetyl-D,
L-phenylalanine. These compounds had good
general antifungal activity and might have potential future agricultural applications.