TITLE:
Safety Properties and Probiotic Potential of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895
AUTHORS:
Ammar AlGburi, Anna Volski, Carla Cugini, Emily M. Walsh, Vladimir A. Chistyakov, Maria S. Mazanko, Anzhelica B. Bren, Leon M. T. Dicks, Michael L. Chikindas
KEYWORDS:
Bacillus, Safety, Probiotics, Antimicrobials, Bacteriocins, Spores
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.6,
May
13,
2016
ABSTRACT: This study reports on the safety and putative probiotic properties of Bacillus amyloliquefaciens B-1895 and Bacillus subtilis KATMIRA1933.
According to the bacterial reverse mutation (Ames) test, cell-free supernatants
of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933
were not mutagenic. The two strains co-aggregated with Escherichia coli and Pseudomonas aeruginosa, and cell-free
supernatants inhibited the growth of Streptococcus intermedius and Porphyromonas
gingivalis. Endospores of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were tolerant to
0.3% (w/v) bile salts and survived incubation
for 4 h in MRS broth at pH 2.0 to 3.0. The ability of the two strains to
produce antimicrobial compounds potentiates their application in health care formulations,
personal care products, food and animal
feed.